Polymerase chain reaction (PCR) is molecular biology based laboratory technique used for amplification of DNA fragment. DNA is amplified by cycles of heating and cooling with enzyme and substrate. Sufficient copies of DNA can be obtained from desired fragment of DNA. Components of PCR Process of PCR This cycle of […]
Biochemistry
Epigenetics is the change made in gene expression without change in DNA sequences. It is reversible change without mutation. It is heritable both mitotically and meiotically. Primary mechanism of epigenetic changes: DNA methylation It is adding methyl group in DNA. It changes the expression of DNA without changing the underlying […]
Also called tri-carboxylic acid (TCA) cycle. The TCA cycle is both catabolic and anabolic. It is catabolic as all macromolecules when broken down enter to TCA cycle for energy production. And it is anabolic as TCA intermediates are used for synthesis of other compounds. Examples: Succinyl Co-A for heme synthesis, […]
Polyunsaturated fatty acids (PUFA) are those fatty acids which have more than 2 double bonds on their structure. These cannot be synthesized inside the body and requirement must be fulfilled through diet. Nuts, seeds, plant oil, sea food are rich in PUFA. Methyl end → CH₃CH₂CH=CHCH₂CH=CHCH₂CH=CH(CH₂)₇COOH ← Carboxy end Numbering […]
